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Turning Tragedy into Triumph Through Walking With Anthony

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On the morning of February 6, 2010, Anthony Purcell took a moment to admire the churning surf before plunging into the waves off Miami Beach. Though he had made the dive numerous times before, that morning was destined to be different when he crashed into a hidden sandbar, sustaining bruises to his C5 and C6 vertebrae and breaking his neck.

“I was completely submerged and unable to rise to the surface,” Purcell recalls. “Fortunately, my cousin Bernie saw what was happening and came to my rescue. He saved my life, but things would never be the same after that dive.”

Like thousands of others who are confronted with a spinal cord injury (SCI), Purcell plunged headlong into long months of hopelessness and despair. Eventually, however, he learned to turn personal tragedy into triumph as he reached out to fellow SCI victims by launching Walking With Anthony.

Living with SCI: the first dark days

Initial rehabilitation for those with SCIs takes an average of three to six months, during which time they must relearn hundreds of fundamental skills and adjust to what feels like an entirely new body. Unfortunately, after 21 days, Purcell’s insurance stopped paying for this essential treatment, even though he had made only minimal improvement in such a short time.

“Insurance companies cover rehab costs for people with back injuries, but not for people with spinal cord injuries,” explains Purcell. “We were practically thrown to the curb. At that time, I was so immobile that I couldn’t even raise my arms to feed myself.”

Instead of giving up, Purcell’s mother chose to battle his SCI with long-term rehab. She enrolled Purcell in Project Walk, a rehabilitation facility located in Carlsbad, California, but one that came with an annual cost of over $100,000.

“My parents paid for rehabilitation treatment for over three years,” says Purcell. “Throughout that time, they taught me the importance of patience, compassion, and unconditional love.”

Yet despite his family’s support, Purcell still struggled. “Those were dark days when I couldn’t bring myself to accept the bleak prognosis ahead of me,” he says. “I faced life in a wheelchair and the never-ending struggle for healthcare access, coverage, and advocacy. I hit my share of low points, and there were times when I seriously contemplated giving up on life altogether.”

Purcell finds a new purpose in helping others with SCIs

After long months of depression and self-doubt, Purcell’s mother determined it was time for her son to find purpose beyond rehabilitation.

“My mom suggested I start Walking With Anthony to show people with spinal cord injuries that they were not alone,” Purcell remarks. “When I began to focus on other people besides myself, I realized that people all around the world with spinal cord injuries were suffering because of restrictions on coverage and healthcare access. The question that plagued me most was, ‘What about the people with spinal cord injuries who cannot afford the cost of rehabilitation?’ I had no idea how they were managing.”

Purcell and his mother knew they wanted to make a difference for other people with SCIs, starting with the creation of grants to help cover essentials like assistive technology and emergency finances. To date, they have helped over 100 SCI patients get back on their feet after suffering a similar life-altering accident.

Purcell demonstrates the power and necessity of rehab for people with SCIs

After targeted rehab, Purcell’s physical and mental health improved drastically. Today, he is able to care for himself, drive his own car, and has even returned to work.

“Thanks to my family’s financial and emotional support, I am making amazing physical improvement,” Purcell comments. “I mustered the strength to rebuild my life and even found the nerve to message Karen, a high school classmate I’d always had a thing for. We reconnected, our friendship evolved into love, and we tied the knot in 2017.”

After all that, Purcell found the drive to push toward one further personal triumph. He married but did not believe a family was in his future. Regardless of his remarkable progress, physicians told him biological children were not an option.

Despite being paralyzed from the chest down, Purcell continued to look for hope. Finally, Dr. Jesse Mills of UCLA Health’s Male Reproductive Medicine department assured Purcell and his wife that the right medical care and in vitro fertilization could make their dream of becoming parents a reality.

“Payton joined our family in the spring of 2023,” Purcell reports. “For so long, I believed my spinal cord injury had taken everything I cared about, but now I am grateful every day. I work to help other people with spinal cord injuries find the same joy and hope. We provide them with access to specialists, funding to pay for innovative treatments, and the desire to move forward with a focus on the future.”

Rosario is from New York and has worked with leading companies like Microsoft as a copy-writer in the past. Now he spends his time writing for readers of BigtimeDaily.com

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Business

Revolutionizing Single-Cell RNA-seq With Automated Cell Counters: Insights From Logos Biosystems

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Despite its relative newness, single-cell RNA sequencing (scRNA-seq) has become an essential component of modern biological research.

 

It can be used to characterize abnormal cell populations, discover and analyze rare cell cellular map networks, and discover subtle yet notable heterogeneities.

scRNA-seq has come a long way since its origins in next-generation sequencers from the late 1990s. While traditional sequencing methods measure a bulk of a cell population to determine its “average genome,” scRNA-seq is significantly more precise. It locates and extracts genomes from individual cells, using each cell to tell part of the genetic story of a greater whole.

Although single-cell sequencing provides valuable information, the process has several drawbacks depending on the method used.

For example, Laser Capture Microdissection (LCM) uses a laser to isolate target cells from a complete solid tissue sample located on a microscope slide. This approach is quick, reliable, and also usable on intact tissues, but it requires the user to identify target cells through visual inspection of their morphology. Cells can also be damaged in the process.

Other methods, like Magnetic-Activated Cell Sorting (MACS) or Fluorescence-Activated Cell Sorting (FACS), come with positives and negatives. Regardless of the approach, advancements in single-cell sequencing technology require significant time and investment, making access to newer and more efficient technologies a barrier to progress.

Overcoming Obstacles With Technological Advancement

When manually counting cells in scRNA-seq analysis, adequately going through each cell can take a huge amount of time and resources. This process also has a high margin of error, sometimes making it difficult to justify the effort.

Moreover, when cell counts are inaccurate for scRNA-seq analysis, overall data quality becomes less reliable, making the research outcomes less reliable and further exacerbating the original issues of time and cost.

With so much time and capital going toward this research, the data it produces should be worthwhile. However, the quality of the data ultimately relies on the quality of the sample before processing, which is where Logos Biosystems and their LUNA-FX7 Automated Cell Counter come in.

Enhancing Research Capabilities

Logos Biosystems is a leader in automated cell counting technology and scRNA-seq analysis and is known for developing the award-winning LUNA Cell Counter family.

Founded in 2008, the company has lived up to its motto of “seeing beyond the cell” by working to improve human health through imaging solutions that help researchers gather quality data in a timely fashion.

Their LUNA series of cell counters specializes in improving research accuracy and efficiency by allowing scientists to spend less time dealing with the monotony of cell counting and more time making valuable observations and implementing solutions.

The LUNA-FX7 Automated Cell Counter gives precise and reliable cell counts. It improves the quality of scRNA-seq analysis and takes less time than other automated counters.

This device has many invaluable features, such as increased size for sample throughput, an expanded cell concentration range, built-in QC software, validation slides for fluorescence, and brightfield to allow for daily QC monitoring and reporting.

Making Research Matter

Incorporating an automated cell counter like the LUNA-FX7 more broadly in scRNA-seq would improve research outcomes and accelerate scientific discoveries. Having machines take care of menial tasks frees up time for scientists and researchers to use their critical and creative thinking skills to push progress in their respective fields forward in ways machines couldn’t do alone.

Applications for automated cell counting technology are almost limitless, including research into developmental studies, immunology, oncology, neurobiology, diabetes, microbiology, and much more. Being able to quickly and precisely profile, identify, classify, and discover rare or new cell types from across the human body allows greater insight into these disciplines and what they can do for human health and growth.

The exciting future of single-cell RNA sequencing lies in the seamless integration of these automated technologies. As they become more widely adopted, they will pave the way for more innovative discoveries that could shape the understanding of biology and medicine. With technologies like Logos Biosystems’ LUNA-FX7, the question of whether the scientific community can see transformative discoveries is now mute as it continues to work to enhance precision and efficiency in cell evaluation, which is vital for scRNA-seq experiments to be successful to not only advance science but also improve human health and well-being on a global scale.

 

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